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Commit 80550444 authored by Hugo CHAUVET's avatar Hugo CHAUVET :bicyclist:
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Revert "port the script for ACAMAS" 

This reverts commit 2fa716f2
parent ef5851f9
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1 merge request!2Revert "port the script for ACAMAS"
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ImageJ/Load_MM_files_Virtual_.py deleted 100644 → 0
+ 0
− 26
View file @ ef5851f9
#@ File (label="Root directory of image", style="directory") rootdir
#@ String (label="file pattern", description="Give the file pattern mydata_{z:d}_{x:003d}_{filter}") filepattern
#@ ConvertService convertService
"""
A way to load any series of .tif in several folders as a virtualstack.
"""
import os
from ij import ImagePlus, VirtualStack, IJ
from net.imglib2.img.display.imagej import ImageJFunctions as IL
import sys
if __name__ == '__main__':
# Add basedir to path
basedir = os.path.dirname(__file__)
sys.path.append(basedir)
# Load the image sequence loader
from utils.ImagesLoaders import load_image_sequence
#filepath = '/home/hugo/Documents/Boulo/ligne_DISCO/20200160/Pn_long_100x_1530tiles_1/'
filepath = rootdir.absolutePath
#path_pattern = 'roi6_tile{tile:d}/img_000000000_{filter}_{z:003d}.tif'
imp, fn = load_image_sequence(filepath, filepattern, return_filenames=True)
imp.show()
ImageJ/Test_imgJ2_ops.py deleted 100644 → 0
+ 0
− 54
View file @ ef5851f9
#@ OpService ops
#@ Dataset data
# to run this tutorial run 'file->Open Samples->Confocal Series' and make sure that
# confocal-series.tif is the active image
from net.imglib2.util import Intervals
from net.imagej.axis import Axes
from net.imglib2.img.display.imagej import ImageJFunctions as IL
from net.imglib2.algorithm.math.ImgMath import computeIntoFloat, sub, div, GT, LT, IF, THEN, ELSE, minimum
from net.imglib2.view import Views
from net.imglib2.converter import Converters
from net.imglib2.img.array import ArrayImgs
from net.imglib2.view import Views
from ij import IJ, ImagePlus
# first take a look at the size and type of each dimension
for d in range(data.numDimensions()):
print "axis d: type: "+str(data.axis(d).type())+" length: "+str(data.dimension(d))
img=data.getImgPlus()
print(img.numDimensions())
print(img.dimensionIndex(Axes.CHANNEL), img.dimensionIndex(Axes.Z), img.dimensionIndex(Axes.TIME))
xLen = data.dimension(data.dimensionIndex(Axes.X))
yLen = data.dimension(data.dimensionIndex(Axes.Y))
zLen = data.dimension(data.dimensionIndex(Axes.Z))
tLen = data.dimension(data.dimensionIndex(Axes.TIME))
cLen = data.dimension(data.dimensionIndex(Axes.CHANNEL))
# crop a channel
c0=ops.transform().crop(img, Intervals.createMinMax(0, 0, 3, 5, 0, xLen-1, yLen-1, 3, 5, tLen-1))
converted = ops.convert().float32(c0)
print(converted.numDimensions())
minvalue = 6
op1 = sub(converted, 750)
op = IF(LT(op1, 0), THEN(minvalue), ELSE(op1))
res = computeIntoFloat(op)
res = ops.convert().uint16(res)
#print(ops.op("stat.min",res))
#IL.show(converted)
aaa=IL.wrap(res, "ImgMath view")
aaa.show()
print(type(aaa))
one = Views.interval(res, Intervals.createMinMax(0, 0, 3, xLen-1, yLen-1, 3))
resa = float(ops.run("stats.max", one).toString())
print(resa)
IL.show(one)
\ No newline at end of file
ImageJ/script_mosaic_telemos.py
+ 58
− 240
View file @ 80550444
......@@ -40,85 +40,12 @@ from utils.ImagesLoaders import load_image_sequence
# Import to do operation with ImageJ2 and ImgMath
from net.imglib2.util import Intervals
from net.imglib2.img.display.imagej import ImageJFunctions as IL
from net.imglib2.algorithm.math.ImgMath import computeIntoFloats, sub, div, GT, LT, IF, THEN, ELSE, minimum
from net.imglib2.algorithm.math.ImgMath import computeIntoFloat, sub, div, GT, LT, IF, THEN, ELSE, minimum
from net.imglib2.type.numeric.real import FloatType
from net.imglib2.view import Views
from net.imagej import Dataset
from net.imagej.axis import Axes
def getFirstMetadata(metadata):
"""
Return the first keys starting with Metadata
"""
ml = list(metadata)
cpt = 0
for l in ml:
cpt += 1
if l.startswith('Metadata'):
break
return cpt
def getMMversion(metadata):
"""
Get MM version from metadata
"""
if int(metadata['Summary']["MicroManagerVersion"].split('.')[0]) >= 2:
version = 2
else:
version = 1
return version
def getRoi(metadata, mm_version=2):
"""
Return the ROI rectangle from metadata file
"""
if mm_version == 2:
# Use the first image to get the ROI
i = getFirstMetadata(metadata)
roi = metadata[metadata.keys()[i]]['ROI']
else:
roi = metadata['Summary']['ROI']
roi = [int(r) for r in roi.split('-')]
return roi
def getScale(metadata):
"""
Return the scale from metadata of MM
"""
try:
# MM2 version
i = getFirstMetadata(metadata)
scale=metadata[metadata.keys()[i]]['PixelSizeUm']
except:
# MM1 version
scale = metadata['Summary']['PixelSize_um']
return scale
def getWidthHeight(metadata):
"""
Get the width and height from metadata
"""
try:
# MM2 version
i = getFirstMetadata(metadata)
height = metadata[metadata.keys()[i]]['Height']
width = metadata[metadata.keys()[i]]['Width']
except:
# MM1 version
height = metadata['Summary']['Height']
width = metadata['Summary']['Width']
return width, height
def load_image_tiles_stack(images_dir):
"""
Fonction pour charge le stack d'images depuis
......@@ -189,20 +116,12 @@ def load_image_tiles_stack(images_dir):
file_pattern = selected_roi+'-Pos_{rows:003d}_{cols:003d}'
mmetapath = os.path.join(base_dir,file_pattern.format(rows=0, cols=0),'metadata.txt')
# Chargement des métadonnées MM (juste le resumée de la premiere tuile suifit)
metadata = json.load(open(mmetapath))
mmversion = getMMversion(metadata)
# Test de version sur MicroManager
if mmversion >= 2:
# Add MM2.0 file structure
file_pattern = os.path.join(file_pattern, 'img_channel{filter}_position{tile-1:003d}_time000000000_z{z:003d}.tif')
hstack, good_names = load_image_sequence(base_dir, file_pattern, return_filenames=True)
else:
# Add MM1.4 file structure
file_pattern = os.path.join(file_pattern, 'img_000000000_{filter}_{z:003d}.tif')
hstack, good_names = load_image_sequence(base_dir, file_pattern, return_filenames=True)
# Chargement des métadonnées MM (juste le resumée de la premiere tuile suifit)
metadata = json.load(open(mmetapath))['Summary']
hstack.setTitle("Data")
......@@ -210,18 +129,13 @@ def load_image_tiles_stack(images_dir):
def load_images(images_dir, roi=None, virtual=False):
d = os.path.abspath(images_dir)
# MM1.4 pattern for white has a pos0
if os.path.isdir(os.path.join(d,'Pos0')):
# MM1.4 pattern for white
white_pattern = os.path.join('Pos0','img_000000000_{filter}_{z:003d}.tif')
else:
# Use MM2 pattern img_channel000_position000_time000000000_z000.tif
white_pattern = os.path.join('Default','img_channel{filter}_position000_time000000000_z{z:003d}.tif')
imp = load_image_sequence(d, white_pattern)
if roi is not None and len(roi) == 4 and imp.width > roi[2] and imp.height > roi[3]:
IJ.log('Roi %s on image size (%i, %i)' % (str(roi[2])+','+str(roi[3]), imp.width, imp.height))
imp.setRoi(*roi)
#IJ.run(imp, "Crop", "")
#IJ.run(img, "Crop", "")
return imp
......@@ -293,7 +207,7 @@ def process_white(implus_white, implus_darkofwhite, zofwhite, roi=None):
# Substract white and dark
opsus = sub(img_whiteC, img_darkC)
whitemindark = computeIntoFloats(opsus)
whitemindark = computeIntoFloat(opsus)
maxwhite = float(ops.run("stats.max", whitemindark).toString())
# Compute the minimum value of the ratio
......@@ -305,7 +219,7 @@ def process_white(implus_white, implus_darkofwhite, zofwhite, roi=None):
THEN(div(opsus,maxwhite)),
ELSE(ratiomin))
result = computeIntoFloats(op)
result = computeIntoFloat(op)
# Convert back to ImagePlus
img_white_corr = IL.wrap(result,
"white corrected from dark (z: %i)" % zofwhite)
......@@ -338,7 +252,7 @@ def process_tiles(implus_tiles_stack, channel, z, implus_white_corr, implus_dark
The stack containing the dark images for each channels
- metadata: dict,
The metadata of MM 1.4 or MM2
The metadata of MM 1.4 (only summary)
return
------
......@@ -351,31 +265,11 @@ def process_tiles(implus_tiles_stack, channel, z, implus_white_corr, implus_dark
# Extract the dark for the color position of the dataset:
# TODO: Fix selecting channel when dark and white does not
# have the same amount of filter
dark = convertService.convert(implus_dark_stack, Dataset)
if dark.numDimensions() > 2:
dark = IL.wrap(implus_dark_stack)
dark = Views.hyperSlice(dark, 2, channel-1)
else:
# Need to apply crop on dark
roi = getRoi(metadata)
if roi is not None and len(roi) == 4 and implus_dark_stack.width > roi[2] and implus_dark_stack.height > roi[3]:
xmin, ymin, xmax, ymax = roi
xmax = xmin + xmax
ymax = ymin + ymax
else:
xmin = ymin = 0
xmax = implus_dark_stack.width
ymax = implus_dark_stack.height
dark = ops.transform().crop(dark,
Intervals.createMinMax(xmin, ymin,
xmax-1, ymax-1))
IJ.log('dark is not an hyperslice, only one dark, keep this one')
# Prepare the white
white = convertService.convert(implus_white_corr, Dataset)
white = IL.wrap(implus_white_corr)
# Extract the zposition and channel of the data-to-procced
data = convertService.convert(implus_tiles_stack, Dataset)
......@@ -402,24 +296,22 @@ def process_tiles(implus_tiles_stack, channel, z, implus_white_corr, implus_dark
if inter is not None:
data = ops.transform().crop(data, inter)
# Operation
op = [None] * Ntiles
cpt = 0
for tile in xrange(Ntiles):
IJ.showProgress(tile, Ntiles)
IJ.log('Compute %i / %i' % (tile, Ntiles))
data_views = Views.hyperSlice(data, 2, tile)
opsus = sub(data_views, dark)
op[cpt] = IF(GT(opsus, 0.0), THEN(div(opsus, white)), ELSE(0.0))
op[cpt] = IF(GT(opsus, 0), THEN(div(opsus, white)), ELSE(0))
cpt += 1
result = datasetService.create(Views.stack([o.view(FloatType()) for o in op]))
result = ops.convert().uint16(result)
# Create the ImagePlus from the result con verted back to uint16
name = metadata['Summary']['ChNames'][channel-1]
name = metadata['ChNames'][channel-1]
imp_title = "Processed Tiles (channel: %i-%s, z: %i)" % (channel, name, z)
img_tiles_corr = IL.wrap(result, imp_title)
# Convert axis to Time and se the resolution
......@@ -443,7 +335,7 @@ def run_processing(channel, zimg, zwhite, run_basic=True, optimizeXY=True, savep
if not os.path.exists(savepath):
os.makedirs(savepath)
chname = metadata['Summary']['ChNames'][channel-1]
chname = metadata['ChNames'][channel-1]
# A folder for the processed tiles
procfolder = savepath+'/DW_processed_tiles_%s_z%i_whitez%i' % (chname, zimg, zwhite)
if not os.path.exists(procfolder):
......@@ -509,9 +401,8 @@ def run_processing(channel, zimg, zwhite, run_basic=True, optimizeXY=True, savep
proctiles.close()
def run_gui(default_values=None):
channels = metadata['Summary']['ChNames']
dataZmax = metadata['Summary']['Slices']
channels = metadata['ChNames']
dataZmax = metadata['Slices']
whiteZmax = white.getNSlices()
if default_values is None:
......@@ -584,32 +475,30 @@ def run_gui(default_values=None):
white.close()
def getMMtiles(metadata, selected_roi, tilesondisk):
def extract_tile_positions(metadata, selected_roi, tilesondisk, tilename='tile_'):
"""
Extract tiles position from micromanager metadata
tilesondisk, list of tiles name found on disk
Extraction des positions des tuiles depuis les métadonnées
pour les rendres compatible avec le plugin de stitching.
"""
mmversion = getMMversion(metadata)
mmsum = metadata['Summary']
xt = None
yt = None
IJ.log("Extract tiles coordinates from metadata")
# Extract pixel size
pix2um = metadata['PixelSize_um']
# The roi width
roiwidth = metadata['Width']
if mmversion == 1:
# Positions
xt = [None] * len(mmsum['InitialPositionList'])
yt = [None] * len(mmsum['InitialPositionList'])
xt = [None] * len(metadata['InitialPositionList'])
yt = [None] * len(metadata['InitialPositionList'])
test_nameSE = 'roi'+selected_roi
test_nameGC = '%s-Pos_' % selected_roi
uniquecols = set(a['GridColumnIndex'] for a in mmsum['InitialPositionList'])
uniquerows = set(a['GridRowIndex'] for a in mmsum['InitialPositionList'])
uniquecols = set(a['GridColumnIndex'] for a in metadata['InitialPositionList'])
uniquerows = set(a['GridRowIndex'] for a in metadata['InitialPositionList'])
Nrow = max(uniquerows) + 1
Ncol = max(uniquecols) + 1
for i, pl in enumerate(mmsum['InitialPositionList']):
for i, pl in enumerate(metadata['InitialPositionList']):
if pl['Label'] in tilesondisk:
# print(pl['Label'])
if test_nameSE in pl['Label']:
......@@ -629,77 +518,11 @@ def getMMtiles(metadata, selected_roi, tilesondisk):
else:
IJ.log("[WARNING]: File %s listed on metadata does not exist on disk" % pl['Label'])
else:
# for MM2
xt = [None] * len(mmsum['StagePositions'])
yt = [None] * len(mmsum['StagePositions'])
test_nameSE = 'roi'+selected_roi
test_nameGC = '%s-Pos_' % selected_roi
uniquecols = set(a['GridCol'] for a in mmsum['StagePositions'])
uniquerows = set(a['GridRow'] for a in mmsum['StagePositions'])
Nrow = max(uniquerows) + 1
Ncol = max(uniquecols) + 1
for i, pl in enumerate(mmsum['StagePositions']):
if pl['Label'] in tilesondisk:
# print(pl['Label'])
if test_nameSE in pl['Label']:
ind = i
if test_nameGC in pl['Label']:
# Compute the globel indice ind = col*(Nrow)+row
# as the order is not the same as the one loaded by bio-format reader
c = pl['GridCol']
r = pl['GridRow']
ind = c * Nrow + r
# print(ind, pl['Label'])
xt[ind] = pl['DevicePositions'][0]['Position_um'][0]
yt[ind] = pl['DevicePositions'][0]['Position_um'][1]
else:
IJ.log("[WARNING]: File %s listed on metadata does not exist on disk" % pl['Label'])
return xt, yt
def extract_tile_positions(metadata, selected_roi, tilesondisk, tilename='tile_'):
"""
Extraction des positions des tuiles depuis les métadonnées
pour les rendres compatible avec le plugin de stitching.
"""
IJ.log("Extract tiles coordinates from metadata")
# Extract pixel size
pix2um = getScale(metadata)
# The roi width
roiwidth, _ = getWidthHeight(metadata)
# Is the microscope use Andor camera ?
i = getFirstMetadata(metadata)
if metadata[metadata.keys()[i]]['Camera'] == 'Andor':
ACAMAS = True
else:
ACAMAS = False
xt, yt = getMMtiles(metadata, selected_roi, tilesondisk)
if ACAMAS:
xtmp = yt
ytmp = xt
xt = xtmp
yt = ytmp
#xt = [-x for x in xtmp]
#yt = [-y for y in ytmp]
# Remove None values in xt and yt (when the tile is not on disk)
igood = [i for i,v in enumerate(xt) if v is not None]
xt = [xt[i] for i in igood]
yt = [yt[i] for i in igood]
if ACAMAS:
xt = [-x for x in xt]
xt = map(lambda x: x/pix2um, xt)
yt = map(lambda x: x/pix2um, yt)
......@@ -709,7 +532,6 @@ def extract_tile_positions(metadata, selected_roi, tilesondisk, tilename='tile_'
xt = [x-xt[0] for x in xt]
yt = [y-yt[0] for y in yt]
outstr = []
for i in range(len(yt)):
outstr += ['%s%04i.tif; ; (%0.2f, %0.2f)'%(tilename, i+1, yt[i], -xt[i])]
......@@ -741,11 +563,7 @@ if __name__ in ['__builtin__','__main__']:
hstack, metadata, rootdir, selectedroi, file_names = load_image_tiles_stack(fdata)
hstack.show()
# GET the version of MM
mmversion = getMMversion(metadata)
# LOAD ROI info
roi = getRoi(metadata, mmversion)
roi = metadata['ROI']
IJ.log('ROI: %s' % str(roi))
# Make a list of tiles names
......
ImageJ/utils/ImagesLoaders.py
+ 153
− 0
View file @ 80550444
# -*- coding: utf-8 -*-
"""
Contain function or class to make import of image sequences easier in ImageJ
"""
import os
from path_tools import find_groups_values
from string import Formatter
from itertools import product
from loci.formats import ChannelSeparator
from ij import ImagePlus, VirtualStack, IJ
def load_image_sequence(rootdir, path_pattern, order=None, channel_group=None,
z_group=None, return_filenames=False):
"""
Load an image sequence (several tiff files in several sub-folders) as an ImageJ VirtualStack.
It uses the python string format to define the pattern of file names
If order is specified as a list of pattern keys, image will be loaded in that order,
otherwise the order are the one in the pattern file names.
Parameters:
-----------
rootdir: string,
The fixed part of the path to image folder and subfolders
path_pattern: string,
The moving part of the folder/file_names.ext where you can use
python string format convention.
order: list of string or None,
The order of pattern keys in which images should be loaded
channel_group: string or None,
The key which contains the color-band dimension of your stack
z_group: string or None,
The key of the z dimension of your stack
return_filenames: boolean,
If true return the list of file names
Examples:
---------
TODO
"""
# Check that path_pattern doest not start with a /
if path_pattern.startswith(os.path.sep):
path_pattern = path_pattern[len(os.path.sep):]
# Find group values
gp_values = find_groups_values(os.path.join(rootdir, path_pattern))
if order is None:
keys = list(i[1] for i in Formatter().parse(path_pattern))
keys_order = []
for k in keys:
if k is not None and k not in keys_order:
keys_order += [k]
else:
keys_order = order
# Set some smath things (if key is c or channel or filter set as channel_group, or if key is z set as z_group)
if z_group is None:
for t in ['z', 'Z']:
if t in keys_order:
z_group = t
break
if channel_group is None:
for t in ['c', 'channel', 'channels', 'filter', 'filters']:
if t in keys_order:
channel_group = t
break
if t.upper() in keys_order:
channel_group = t.upper()
break
if t.capitalize() in keys_order:
channel_group = t.capitalize()
break
# Z should be before channel group but at the end (if given for hyperstack dimension)
if z_group is not None:
keys_order.remove(z_group)
keys_order += [z_group]
# If a channel group is given (need to be at the end of keys_order)
if channel_group is not None:
keys_order.remove(channel_group)
keys_order += [channel_group]
# Create the combination of all possible groups values
allpattern_values = list(product(*(gp_values[k] for k in keys_order)))
# Generate the list of file names to load
allnames = tuple(path_pattern.format(**dict(zip(keys_order, p))) for p in allpattern_values)
# We need to load one image to get it's size
fr = ChannelSeparator()
fr.setGroupFiles(False)
fr.setId(os.path.join(rootdir, allnames[0]))
metadata = fr.getGlobalMetadata()
width, height = fr.getSizeX(), fr.getSizeY()
# Create a virtualStack
vstack = VirtualStack(width, height, None, rootdir)
for imgname in allnames:
vstack.addSlice(imgname)
# Create the ImagePlus to display the stack
imp = ImagePlus("%s" % rootdir, vstack)
# Set the dimension of hyperstack if keys for color channels
# and z channels are given
if channel_group is not None:
nchannel = len(gp_values[channel_group])
else:
nchannel = 1
if z_group is not None:
nslice = len(gp_values[z_group])
else:
nslice = 1
# Compute the time from the product of not used channel
not_used_keys = []
for k in keys_order:
if k != channel_group and k != z_group:
not_used_keys += [k]
ntimes = 1
for k in not_used_keys:
ntimes *= len(gp_values[k])
imp.setDimensions(nchannel, nslice, ntimes)
imp.setOpenAsHyperStack(True)
# Try to set the physical size from Metadata
try:
IJ.run(imp,
"Properties...",
"pixel_width={resox} pixel_height={resoy} pixel_unit='um'".format(resox=1/metadata['XResolution'],
resoy=1/metadata['YResolution']));
except:
pass
# Set auto contrast
IJ.run(imp, "Enhance Contrast", "saturated=0.35");
if return_filenames:
return imp, allnames
else:
return imp
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